1% BSA (Bovine Serum Albumin) Wash - ml  


1% bovine serum albumin in 1X phosphate buffered saline - pH 7.4 and 0.1% Triton-X-100

Reagents needed:

5
g bovine serum albumin (BSA)
2.5
50
ml ddH2O

Directions:

1) Dissolve 5 g powered BSA into 440 ml of ddH2O by stirring.

2) Add 50 ml of 10X PBS while stirring.

3) Add 2.5 ml of 20% Triton-X-100.

4) Add ddH2O until final volume is 500 ml.

5) Filter 1% BSA Wash Buffer through a 0.45 μm filter.

6) Aliquot and freeze at -20 °C for long-term storage.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com