1% bovine serum albumin in 1X phosphate buffered saline - pH 7.4 and 0.1% Triton-X-100
Reagents needed:
5 | g bovine serum albumin (BSA) |
2.5 | |
50 | ml 10X PBS |
440 | ml ddH2O |
Directions:
1) Dissolve 5 g powered BSA into 440 ml of ddH2O by stirring.
2) Add 50 ml of 10X PBS while stirring.
3) Add 2.5 ml of 20% Triton-X-100.
4) Add ddH2O until final volume is 500 ml.
5) Filter 1% BSA Wash Buffer through a 0.45 μm filter.
6) Aliquot and freeze at -20 °C for long-term storage.
© 2007 Joseph T.E. Roland
www.Cytographica.com